Fluorescence turn-on detection of a protein through the displaced single-stranded DNA binding protein binding to a molecular beacon.
نویسندگان
چکیده
A new approach has been developed for the highly sensitive and selective sensing of a protein. Lysozyme binding to its aptamer prevents SSB protein binding, and the subsequent binding of the free SSB protein to a molecular beacon results in a turn-on fluorescence signal, which can be used for lysozyme quantification.
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ورودعنوان ژورنال:
- Chemical communications
دوره 47 19 شماره
صفحات -
تاریخ انتشار 2011